Antifibrotic Potential of Nigella Sativa Extract: Study on Male Wistar White Rats Induced by Hepatotoxic Agents

EasyChair Preprint 10856

Abstract

Liver fibrosis is the initial stage of the liver injury response, which is still reversible. Continued injury causes damage to hepatocytes, activating Hepatic Stellate Cells to become myofibroblasts, producing type 1 collagen. Untreated collagen type 1 and fibril tissue piles can lead to liver cirrhosis. The administration of herbal antifibrotics has become the focus of current research. One herbal treatment that has antifibrotic functions is black cumin seeds. This study aimed to prove the effect of black seed extract (Nigella sativa) on decreasing expression of collagen type 1 in the liver parenchyma of male Wistar white rats (Rattus norvegicus) liver fibrosis model. The method in this study was True Experimental, Post Test Only Control Group Design with one positive control group and one negative control group, as well as three papaya leaf extract treatment groups, namely doses of 1.2 g/kg, 2.4 g/kg, and 4. 8 g/kgBW. The One Way Anova test results showed a significant difference (sig=0.000). The Post-Hoc Tukey test results showed significant differences between each treatment group. The correlation test results showed a strong relationship, namely a correlation coefficient value of -0.978. The regression test yielded 95.3% results, which means that black cumin extract (Nigella sativa) had a 95.3% effect on the expression of type 1 collagen in the liver parenchyma of male Wistar white rats so that the administration of black cumin extract (Nigella sativa) affected decreasing the expression of type 1 collagen liver parenchyma of male white Wistar rat (Rattus norvegicus) liver fibrosis model

Keyphrases: Antifibrotic, Black cumin, Collagen type 1, Hepatic fibrosis, Hepatotoxic agent, Nigella sative, hepatotoxic, liver fibrosis

@booklet{EasyChair:10856,
  author    = {Fathiyah Safithri and Tubagus Arif Hidayatullah and Dian Yuliartha Lestari and Aji Muthi'Ah Nur Azizah},
  title     = {Antifibrotic Potential of Nigella Sativa Extract: Study on Male Wistar White Rats Induced by Hepatotoxic Agents},
  howpublished = {EasyChair Preprint 10856},
  year      = {EasyChair, 2023}}